Cancer

Download Cancer Cell Culture: Methods and Protocols by Ian A. Cree (auth.), Ian A. Cree (eds.) PDF

By Ian A. Cree (auth.), Ian A. Cree (eds.)

With many fresh advances, melanoma cellphone tradition learn is extra very important than ever prior to. This well timed version of Cancer cellphone tradition: tools and Protocols covers the elemental strategies of melanoma cellphone biology and tradition whereas increasing upon the hot shift in mobile tradition equipment from the iteration of latest mobile strains to using basic cells. there are ways to represent and authenticate phone strains, to isolate and advance particular forms of melanoma cells, and to strengthen new mobilephone line versions. useful assays are supplied for the overview of clonogenicity, mobile proliferation, apoptosis, adhesion, migration, invasion, senescence, angiogenesis, and phone cycle parameters. different tools let the amendment of cells for transfection, drug resistance, immortalization, and move in vivo, the co-culture of alternative telephone kinds, and the detection and remedy of illness. during this re-creation, particular emphasis is put on secure operating perform for either cells and laboratory researchers. those chapters comprise the data severe to luck – merely via reliable perform and quality controls will the result of melanoma telephone tradition enhance. Written within the profitable Methods in Molecular Biology™ sequence structure, chapters contain introductions to their respective subject matters, lists of the mandatory fabrics and reagents, step by step, without problems reproducible protocols, and notes on troubleshooting and warding off identified pitfalls.

Authoritative and obtainable, Cancer mobile tradition: equipment and Protocols serves as a pragmatic advisor for scientists of all backgrounds and goals to exhibit the right experience of fascination linked to this examine field.

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Cancer Cell Culture: Methods and Protocols

With many fresh advances, melanoma telephone tradition learn is extra very important than ever earlier than. This well timed variation of melanoma mobilephone tradition: tools and Protocols covers the elemental strategies of melanoma telephone biology and tradition whereas increasing upon the new shift in cellphone tradition tools from the new release of recent cellphone traces to using basic cells.

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And Masui, H. (1980) Hormonal control of human colon carcinoma cell growth in serum-free medium, Proc Natl Acad Sci USA 77, 3464–3468. 14. Evan, G. , Wyllie, A. , Gilbert, C. , Littlewood, T. , Waters, C. , Penn, L. , and Hancock, D. C. (1992) Induction of apoptosis in fibroblasts by c-myc protein, Cell 69, 119–128. 15. Harrington, E. , Bennett, M. , and Evan, G. I. (1994) c-Myc-induced apoptosis in fibroblasts is inhibited by specific cytokines, EMBO J 13, 3286–3295. 16. , Knight, L. , and Cree, I.

3. Contamination Microbial contamination (bacteria, fungi, Mycoplasma, and viruses) of cell culture systems continues to be problematic. In a majority of cases, microbial contamination is overt and is visible with the naked eye. However, low levels of contamination of the more fastidious microorganisms are often missed by many observers. By including a series of microbiological tests, most common microorganisms can be detected. e. blood agar, thioglycollate, trypticase soy, brain heart infusion, Sabouraud, and YM broths plus nutrient broths with 2% yeast extract) tests incubated at 37 and 26°C, under aerobic and anaerobic conditions, will detect the most fastidious organisms known to infect cell cultures and media (6).

W. (1967) Study of a virus-containing hematopoietic cell line and a melanoma cell line derived from a patient with a leukemoid reaction, Lab Invest 16, 882–891. 5. Moore, G. , and Glick, J. L. (1967) Perspective of human cell culture, Surg Clin North Am 47, 1315–1324. 6. Bottenstein, J. , and Sato, G. H. (1979) Growth of a rat neuroblastoma cell line in serum-free supplemented medium, Proc Natl Acad Sci USA 76, 514–517. 7. Sato, J. , Cao, H. , Cabot, M. , Sato, G. , and Welsh, C. J. (1988) Effects of proximate cholesterol ­precursors and steroid hormones on mouse myeloma growth in serum-free medium, In Vitro Cell Dev Biol 24, 1223–1228.

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