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The percentage of particular LMM-Al species varies with the total concentration of Al in serum. Other LMM constituents of blood serum such as lactate, oxalate and amino acids have significantly lower affinities for Al3+ than citrate and phosphate and were therefore not considered in modeling calculations [29]. The presence of Al(OH)4 − was 29 predicted in human serum but at a very low concentration, representing only about 3% of total LMM-Al species [27]. 3 ANALYTICAL TECHNIQUES AND CHEMICAL SPECIATION OF ALUMINUM IN HUMAN SERUM The main problem in chemical speciation of Al in the serum of healthy subjects is the very low Al concentration (a few ng cm−3 ) [12, 13] and contamination by extraneous Al.
Milk is also low in Al, but dairy products, especially some types of cheese, contain up to 70 µg g−1 Al, due to additives used in cheese processing. Cocoa and cocoa products may contain slightly elevated Al concentrations, but coffee powder is low in Al content. 35 µg cm−3 ). Black tea leaves are the foodstuffs with the highest concentrations of Al, ranging from 600 up to 1,300 µg g−1 . 2 µg cm−3 ) are also found in black tea infusions. Beverages in general contain below 8 µg cm−3 Al, though exceptions are some types of red wine with Al concentrations up to 38 µg cm−3 .
34] critically appraised the speciation of Al in serum by ultrafiltration and highlighted the problems of contamination that led to controversial results reported by previous investigators. The authors compared ultrafiltration and microultrafiltration procedures. Al in filtrates was determined by electrothermal atomic absorption spectrometry (ETAAS). Since the classical ultrafiltration procedure was more liable to the contamination with extraneous Al, microultrafiltration, which minimized contamination risk, gave more reliable results.