By P. Vasa, D. Mathur (auth.)
This publication provides rising modern optical ideas of ultrafast technology that have opened completely new vistas for probing organic entities and strategies. The spectrum reaches from time-resolved imaging and multiphoton microscopy to melanoma remedy and stories of DNA harm. The booklet monitors interdisciplinary examine on the interface of physics and biology. rising themes at the horizon also are mentioned, just like the use of squeezed mild, frequency combs and terahertz imaging because the hazard of mimicking organic platforms. The publication is written in a fashion to make it easily available to researchers, postgraduate biologists, chemists, engineers, and physicists and scholars of optics, biomedical optics, photonics and biotechnology.
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Szip˝ocs, Opt. Lett. 22, 528 (1997) 70. M. Nisoli, S. De Silvestri, O. Svelto, R. Szip˝ocs, K. , Opt. Lett. 22, 522 (1997) 71. J. -C. Diels, J. Opt. Soc. Am. B 18, 1218 (2001) 72. K. A. A. Rajgara, D. Mathur, Opt. Express 16, 7083 (2008) 73. B. Piglosiewicz, D. Sadiq, M. Mascheck, S. Schmidt, M. , Opt. Express 19, 14451 (2011) Chapter 3 Nonlinear Microscopy Abstract An overview is presented of the application of ultrafast nonlinear optics to biophotonic microscopy, with the essential physics being based on multiphoton excitation.
It involves the overlapping of two pulse replicas in a nonlinear 34 2 Ultrashort Pulses and Nonlinear Optics: Nuts and Bolts medium (generally a SHG crystal) and recording the generated nonlinear signal as a function of the delay between the two replicas. Typically, a collinear geometry is used to avoid loss of temporal resolution via geometric effects (finite beam angles). The temporal resolution in autocorrelation is limited by the response of the nonlinear material. Since parametric nonlinear processes are essentially instantaneous, this technique has been used successfully even for single-cycle pulses.
Apart from SPM, a collection of other third-order nonlinear processes like self-steepening, cross-phase modulation and stimulated Raman scattering also induce substantial spectral broadening upon intense pulse propagation. The visually prominent manifestation of these phenomena is known as supercontinuum (SC) generation [23, 33–35]. For investigations of SC generation in transparent liquid media, water is a natural choice due to its importance in biological systems [36, 37]. A typical SC spectrum generated in water by intense, IR ultrashort pulses is shown in Fig.